in vivo実験用にオリゴを注文する際に考慮すべきいくつかの重要なポイントをご説明します。
35 result(s)
Gene editing by CRISPR has led to significant advances in immunotherapy development. Here we offer several tips for successful editing in T cells, including recommendations for cell handling, nucleofection, and analysis.
CRISPRテクノロジーによりゲノム編集細胞集団を作製後、クローン細胞株を用いるかプール細胞集団を用いるかを決定する際に考慮すべき最も重要な4つの事項をご説明しています。
Examples of studies using siRNAs targeting lncRNAs to inspire your next experiment.
Isolation of genomic DNA from formalin fixed paraffin embedded (FFPE) tissues is a critical step for molecular diagnostic (MDx) assays. Here we investigate extraction of DNA from FFPE using five comparable methods.
Are you new to RNAi experiments and need some guidance on troubleshooting?
RNAiを成功させるためのヒントとコツ：トランスフェクション時に必要なコントロールを含めてください！
After all the hard work of editing your cell line, you want to have confidence in your new research model. So, how do you verify your cell line is what you expect it to be? Could a heterogeneous cell population be obscuring your editing effects? Is observed phenotype being caused by the targeted gene edit, or unintended off-target effects? Here we discus ways to add supporting data to validate your gene-engineering projects.
The only self-delivering siRNA for difficult-to-transfect cells carries a functional guarantee.
CRISPRテクノロジーは、遺伝子と分子パスウェイをより明確に調べることを可能にしました。どのように、ノックアウト細胞株を、遺伝子レスキューと疾患変異モデルとともに、または独立した細胞モデルとして使用して、研究を検証し、研究成果を拡張できるかを説明しています。